T. Nazari; M. Baranimotlgh; E. Dordipour; R. Ghorbani nasrabadi; S. Sefidgar Shahkolaee
Abstract
Introduction One of the most important needs in crop planning is the evaluation of different systems of plant nutrition. plant nutrition in a correct way can preserve the environment and increase efficiency of agricultural inputs. Humic acid contains many nutrients that increase the soil fertility and ...
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Introduction One of the most important needs in crop planning is the evaluation of different systems of plant nutrition. plant nutrition in a correct way can preserve the environment and increase efficiency of agricultural inputs. Humic acid contains many nutrients that increase the soil fertility and soil organic matter content and thus affect plant growth and yield. In the present study, the effect of soil and foliar application as well as fertigation application of humic acid on iron and phosphorous availability of canola (Hyola 308) was evaluated. Materials and Methods The soil was air-dried and ground to pass through a 2-mm sieve and then was analyzed to determine soil physical and chemical properties using standard methods. A greenhouse experiment was carried out with 10 treatments in four replications. Treatments include humic acid soil application at three levels (1, 2 and 4 g.kg-1 soil), foliar application at three levels (0.1, 0.2 and 0.4%) and three fertigation levels (1000, 2000 and 4000 mg L-1) and control). Soil application in the form of humic acid powder and in cropping time based on the soil weight of the pots and for spraying and use along with irrigation water, each of the spraying and together with irrigation water levels is divided into three equal parts and in three stages (plant establishment, stem elongation and flowering). At the end of growth period (for 139 days), vegetative growth indices were determined and then plants were harvested and stem and leaves were separated. Phosphorous content in plant extracts was measured by molybdate vanadate method (yellow method) and iron concentration in the samples was determined by atomic absorption (AAS-Unicam-919). Statistical analysis was conducted via SAS software and mean comparisons carried out by LSD test at 5% probability level. Results and Discussion Results show that the application methods and the different levels of humic acid had significant impacts (p < 0.01) on the all parameters but they had no significant effects on the numbers of leaves. The maximum leaf fresh weight was 4.34 gr per plant which obtained water irrigated treatments with 2000 mg/lit. However, there was no significant difference between water irrigation with 4000 and 2000 mg/lit humic acid concentrations. Besides, maximum leaf dry weight was 0.37 gr in the plant that was water irrigated with 4000 mg/lit, however there was no significant difference between water irrigation with 4000 and 2000 mg/lit humic acid concentrations. Also, the maximum stem dry and fresh weight was 5.92 and 1.53 gr which observed in water irrigated with 2000 mg/lit. The application methods and the different levels of humic acid had significant impact (p<0.01) on the content and absorption of Fe and P excluding the Fe content in the root. The maximum Fe content in stem and seed were 321.25 and 85 mg/kg was observed in the treatment of humic acid with water irrigation of 2000 mg/lit. Also, in the treatment of spraying with 0.4 % of humic acid, the maximum of Fe concentration (245.46 mg/kg) was obtained. The humic acid molecules can pass from the cell membranes and cause iron reduction in the Apoplast and increase the availability of Fe. Because of the reduction effect of humic acid on the availability and accumulation of Fe in the plant tissue, increasing in the Fe absorption with humic acid treatment is observed. The increase in the accumulation of Fe by humic material might be due to the releasing phenolic material in the root rhizosphere. The maximum P concentration in the leaf, stem and seed were 0.40, 0.72 and 0.897 respectively that was observed in the treatment of water irrigation with 2000 mg/lit with humic acid. Due to the availability of phosphorus and other nutrients for wheat, humic acid increases the plant yield in the reproductive stage of seeding. Conclusion Results of the study show that humic acid can ameliorate the negative effect of a large amount of phosphorus on iron availability. In fact, humic acid, due to providing nutritional balance for the plant, prevents negative effects caused by the high amounts of specific elements such as phosphorus. Also, the results showed that all three humic acid application methods increased vegetative growth parameters, and iron and phosphate availability for canola plant compared to control. But in general, 2000 mg/L acid humic with irrigation water was more effective than two methods of soil and foliar application.
Soil Chemistry and Pollution
Somayeh Sefidgar shahkolaie; Mojtaba Baranimotlagh; Farhad Khormali; Esmael Dordipour
Abstract
Introduction At present, contamination of water and soil resources is an important environmental challenge. Therefore, decontamination of such is a prerequirement for using these resources. Cadmium (Cd) and lead (Pb) often coexist in contaminated soils and there is currently no effective means for their ...
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Introduction At present, contamination of water and soil resources is an important environmental challenge. Therefore, decontamination of such is a prerequirement for using these resources. Cadmium (Cd) and lead (Pb) often coexist in contaminated soils and there is currently no effective means for their concurrent removal. Concerns about their mobility and bioavailability have increased because of food safety, potential health risks and its detrimental effects on the ecosystems. The stabilization/solidification is a cost effective remediation method that prevents spreading of heavy metals in soil and water resources. In this process, contaminated soil reacts with amendments such as organic and liming materials to form low soluble or non-soluble stable materials. The objective of this study was to evaluate the effect of several low cost amendments on Cd and Pb stabilization by a sequential extraction method. Materials and Methods In this research, in order to investigate the effect of organic amendments (biochar 640°C, and biochar 420°C) and inorganic amendments (Pumice, Leca, Zeolite, and Bentonite) on Pb and Cd stabilization in a contaminated soil, an incubation experiment was carried out. One kilogram of each amended soil and the control soil were packed into respective pots. Soils were amended in the laboratory using biochar 640 (BI1), biochar 420 (BI2) bentonite (BE), pumice (P), leca (LE), and zeolite (Z). A control treatment (C) without adding amendment was also prepared. The amendment materials were applied at 1 and 5 percent wt. Each treatment was performed in three replicates and the samples were incubated in the dark at 14°C for 6 months. At the end of the incubation time, the potential bioavailability of Cd in non-amended and amended soils was evaluated by extraction with DTPA and ethylenediamine tetraacetic acid (EDTA). Total Cd (CdT) and Pb (PbT) was extracted by aqua regia (HNO3 + HCl) extraction. The chemical fractions of Cd and Pb were determined by a sequential extraction method which is a five-step chemical fractionation based on the work of Tessier et al. (1979). All statistical analyses were performed using SAS software. Means of different treatments were compared using LSD (P ≤0.05) test. Results and Discussion The results indicated that the additions of amendments to soils reduced the concentration of DTPA and EDTA-extracted Pb and Cd. The smallest concentration of Pb-extracted DTPA and EDTA was observed in organic amendments treated soil (biochar 640°C, and biochar 420°C) and treated with 5% biochar 640°C, respectively. The high sorbent capacity of the BI used in this study could be due to its high pH, high content of organic carbon and cation exchange capacity (CEC). The highest decreasing rate of DTPA and EDTA-extractable of Cd was observed in treated with 5% pumice and zeolite, respectively. Application of the amendments (except for 1% LE) decreased exchangeable fraction (F1) of Pb compared to the non-amended soil. Also, the amendments (except for 1% P, Z and BE) decreased exchangeable fraction (F1) of Cd compared to the non-amended soil. Although the biochar 640 (5%) showed the highest decreasing rate of exchangeable fraction (F1) of Pb and Cd, they increased the oxide (F3) and organic (F4) fractions, which might be due to its rich O-containing functional groups and high alkalinity leading to an increase in the binding of Cd and Pb to organic compounds and mineral oxides. Conclusion Results indicated that application of amendments was successful in lowering the potential bioavailability of Pb and Cd soils. The 5% biochar 640 treatment had the greatest decrease in extractable Pb. Also, the 5% zeolite and pumice treatment had the greatest decrease in extractable Cd. Application of BI resulted in a significant decrease in both Pb and Cd exchangeable fraction (F1). This reduction in the exchangeable fraction (F1) of Cd and Pb in the soil was due to an increase in the fraction of heavy metals bound to the soil organic matter (F4) oxides (F3) after BI addition. Enhanced precipitation or co-precipitation and complexation of metals with amendments led to the reduction of the solubility of the metals. The P, LE, BE, and Z altered the exchangeable fraction (F1) of Cd and Pb to the oxide fraction (F3) and the carbonate fraction (F3), respectively. Application of BI amendment causes the highest decreasing rate of solubility Cd and Pb, suggesting this as the suitable amendment for the remediation of Cd and Pb in contaminated soils.
